pcDNA3-PhoDer
(Plasmid
#188473)
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PurposeExpresses the optogenetic human Gasdermin D (PhoDer) in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 188473 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
- Backbone size w/o insert (bp) 5500
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePhoDer
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SpeciesH. sapiens (human), Synthetic
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Insert Size (bp)2184
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Entrez GeneGSDMD (a.k.a. DF5L, DFNA5L, FKSG10, GSDMDC1)
- Promoter CMV IE
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV
- 3′ sequencing primer BGH (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-PhoDer was a gift from Gary Mo (Addgene plasmid # 188473 ; http://n2t.net/addgene:188473 ; RRID:Addgene_188473) -
For your References section:
Gasdermin D pores are dynamically regulated by local phosphoinositide circuitry. Santa Cruz Garcia AB, Schnur KP, Malik AB, Mo GCH. Nat Commun. 2022 Jan 10;13(1):52. doi: 10.1038/s41467-021-27692-9. 10.1038/s41467-021-27692-9 PubMed 35013201
