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Addgene

pN251
(Plasmid #188471)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188471 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pY108 (gift from Dr. Feng Zhang lab)
  • Vector type
    Mammalian Expression, Bacterial Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    enCas12a-HF (gift from Dr. Keith Joung)
  • Species
    Acidaminococcus sp.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pN251 was a gift from Le Cong (Addgene plasmid # 188471 ; http://n2t.net/addgene:188471 ; RRID:Addgene_188471)

  • For your References section:

    Machine-learning-optimized Cas12a barcoding enables the recovery of single-cell lineages and transcriptional profiles. Hughes NW, Qu Y, Zhang J, Tang W, Pierce J, Wang C, Agrawal A, Morri M, Neff N, Winslow MM, Wang M, Cong L. Mol Cell. 2022 Jun 22. pii: S1097-2765(22)00537-8. doi: 10.1016/j.molcel.2022.06.001. 10.1016/j.molcel.2022.06.001 PubMed 35752172
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