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His_Naa80d in pTYB12
(Plasmid #188455)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188455 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTYB12
  • Backbone manufacturer
    NEB
  • Backbone size w/o insert (bp) 7376
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Naa80
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    717
  • Mutation
    WT
  • Entrez Gene
    NAA80 (a.k.a. FUS-2, FUS2, HsNAAA80, NAT6)
  • Promoter T7
  • Tag / Fusion Protein
    • intein-CBD, 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7_for
  • 3′ sequencing primer T7_term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His_Naa80d in pTYB12 was a gift from Roberto Dominguez (Addgene plasmid # 188455 ; http://n2t.net/addgene:188455 ; RRID:Addgene_188455)

  • For your References section:

    A solution to the long-standing problem of actin expression and purification. Ceron RH, Carman PJ, Rebowski G, Boczkowska M, Heuckeroth RO, Dominguez R. Proc Natl Acad Sci U S A. 2022 Oct 11;119(41):e2209150119. doi: 10.1073/pnas.2209150119. Epub 2022 Oct 5. 10.1073/pnas.2209150119 PubMed 36197995
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