His-FLAG-Tev_ACTB (beta-actin)
(Plasmid #188453)

• Purpose: Express in mammalian cells human beta-actin (ACTB) with N-terminal 6xHis tag, FLAG tag, and TEV protease site.
• Depositing Lab: Roberto Dominguez
• Publication: Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;119(41):e2209150119. doi: 10.1073/pnas.2209150119. Epub 2022 Oct 5.

Backbone

• Vector backbone: pJCX4
• Backbone size w/o insert (bp): 4672
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ACTB
• Alt name: beta cytoplasmic actin
• Species: H. sapiens (human)
• Insert Size (bp): 1122
• Mutation: WT
• Entrez Gene: ACTB (a.k.a. BKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8)
• Promoter: CMV
• Tag / Fusion Protein:
  • 6xHis-FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV_for
• 3′ sequencing primer: WPRE_rev

Resource Information

• Supplemental Documents:
  • hisflagtev_actb-in-pjc.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry