His-FLAG-Tev_ACTA1 (alpha-actin)
(Plasmid #188452)

• Purpose: Express in mammalian cells human alpha-actin (ACTA1) with N-terminal 6xHis tag, FLAG tag, and TEV protease site.
• Depositing Lab: Roberto Dominguez
• Publication: Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;119(41):e2209150119. doi: 10.1073/pnas.2209150119. Epub 2022 Oct 5.

Backbone

• Vector backbone: pJCX4
• Backbone size w/o insert (bp): 4672
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ACTA1
• Alt name: alpha skeletal actin
• Species: H. sapiens (human)
• Insert Size (bp): 1125
• Mutation: WT
• Entrez Gene: ACTA1 (a.k.a. ACTA, ASMA, CFTD, CFTD1, CFTDM, CMYP2A, CMYP2B, CMYP2C, MPFD, NEM1, NEM2, NEM3, SHPM)
• Promoter: CMV
• Tag / Fusion Protein:
  • 6xHis-FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV_for
• 3′ sequencing primer: WPRE_rev

Resource Information

• Supplemental Documents:
  • hisflagtev_acta1-in-pjc.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry