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Addgene

pScalps eSpCas9_Zsgreen gRNA scaffold
(Plasmid #188449)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188449 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pScalps Puro
  • Backbone size (bp) 7743
  • Vector type
    Lentiviral, CRISPR
  • Promoter EF1-alpha

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Introduce sgRNA in lentiviral vector (pScalps eSpCas9_Zsgreen gRNA scaffold) using BsmBI site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pScalps eSpCas9_Zsgreen gRNA scaffold was a gift from Ajit Chande (Addgene plasmid # 188449 ; http://n2t.net/addgene:188449 ; RRID:Addgene_188449)

  • For your References section:

    Improved loss-of-function CRISPR-Cas9 genome editing in human cells concomitant with inhibition of TGF-beta signaling. Mishra T, Bhardwaj V, Ahuja N, Gadgil P, Ramdas P, Shukla S, Chande A. Mol Ther Nucleic Acids. 2022 Mar 8;28:202-218. doi: 10.1016/j.omtn.2022.03.003. eCollection 2022 Jun 14. 10.1016/j.omtn.2022.03.003 PubMed 35402072
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