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Addgene

pcDNA 3.1 BS (-) N protein
(Plasmid #188448)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188448 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA 3.1 BS (-)
  • Backbone size w/o insert (bp) 5427
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 N protein
  • Species
    SARS-CoV-2
  • Insert Size (bp)
    1377
  • Promoter CMV
  • Tag / Fusion Protein
    • Strep-Tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA 3.1 BS (-) N protein was a gift from Ajit Chande (Addgene plasmid # 188448 ; http://n2t.net/addgene:188448 ; RRID:Addgene_188448)

  • For your References section:

    SARS-CoV-2 spike E156G/Delta157-158 mutations contribute to increased infectivity and immune escape. Mishra T, Dalavi R, Joshi G, Kumar A, Pandey P, Shukla S, Mishra RK, Chande A. Life Sci Alliance. 2022 Mar 16;5(7). pii: 5/7/e202201415. doi: 10.26508/lsa.202201415. Print 2022 Jul. 10.26508/lsa.202201415 PubMed 35296517
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