pAG416GPD-Cerulean-VPS21
(Plasmid #18842)

• Depositing Lab: Susan Lindquist
• Publication: Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 105(1):145-50.

Backbone

• Vector backbone: pAG416GPD
• Backbone manufacturer: pRS416
• Backbone size w/o insert (bp): 7497
• Vector type: Yeast Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: VPS21
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 632
• Entrez Gene: VPS21 (a.k.a. YOR089C, VPS12, VPT12, YPT21, YPT51)
• Tag / Fusion Protein:
  • Cerulean (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: GATEWAY CLONING (not destroyed)
• 3′ cloning site: GATEWAY CLONING (not destroyed)
• 5′ sequencing primer: EGFP-C

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry