YPET-GGA3
(Plasmid #18841)

• Depositing Lab: Martin Schwartz
• Publication: Hall et al Anal Biochem. 2008 Mar 15. 374(2):243-9.

Backbone

• Vector backbone: pEF4-myc-HisA
• Backbone manufacturer: In vitrogen
• Backbone size w/o insert (bp): 5882
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: YPET-GGA3
• Species: H. sapiens (human)
• Insert Size (bp): 1209
• Mutation: The fluorescent protein YPET was added to the amino terminus of amino acids 148-303 from GGA3.
• Entrez Gene: GGA3
• Tags / Fusion Proteins:
  • myc (C terminal on backbone)
  • His6 (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry