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pDR111_mannose_Stat1Klf6
(Plasmid #188400)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188400 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDR111
  • Backbone size w/o insert (bp) 8858
  • Total vector size (bp) 12312
  • Modifications to backbone
    Addition of insert and fusion components to NheI site. Phyper-spank replaced by D-mannose promoter and lacI replaced by mannose regulator
  • Vector type
    Bacterial Expression
  • Selectable markers
    Spectinomycin for Bacillus subtilis

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Stat-1
  • Species
    M. musculus (mouse); Escherichia coli
  • Insert Size (bp)
    2250
  • Entrez Gene
    Stat1 (a.k.a. 2010005J02Rik)
  • Promoter D-mannose
  • Tag / Fusion Protein
    • PhoD secretion peptide (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site EagI (not destroyed)
  • 5′ sequencing primer cggctagcTTACAGATGCCTT
  • 3′ sequencing primer TACCTCCTTgtcgactAAGCTT
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Klf6
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    850
  • Entrez Gene
    Klf6 (a.k.a. AI448727, BCD1, C86813, CPBP, Copeb, FM2, FM6, Ierepo1, Ierepo3, R75280, Zf9)
  • Promoter D-mannose
  • Tag / Fusion Protein
    • PhoD secretion peptide

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site EagI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer cggctagcTTACAGATGCCTT
  • 3′ sequencing primer TACCTCCTTgtcgactAAGCTT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDR111_mannose_Stat1Klf6 was a gift from Christopher Contag (Addgene plasmid # 188400 ; http://n2t.net/addgene:188400 ; RRID:Addgene_188400)

  • For your References section:

    Engineered endosymbionts that alter mammalian cell surface marker, cytokine and chemokine expression. Madsen CS, Makela AV, Greeson EM, Hardy JW, Contag CH. Commun Biol. 2022 Aug 30;5(1):888. doi: 10.1038/s42003-022-03851-6. 10.1038/s42003-022-03851-6 PubMed 36042261
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