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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 18840 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEF4-myc-HisA
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5882
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Vector typeMammalian Expression
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCyPET-Arf6
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SpeciesH. sapiens (human)
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Insert Size (bp)1299
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MutationThe sequence for the fluorescent protein CyPET was inserted into human Arf6 between amino acid residues 143 and 144, adding 6 amino acid linkers.
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Entrez GeneARF6
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CyPET-Arf6 was a gift from Martin Schwartz (Addgene plasmid # 18840 ; http://n2t.net/addgene:18840 ; RRID:Addgene_18840) -
For your References section:
A fluorescence resonance energy transfer activation sensor for Arf6. Hall B, McLean MA, Davis K, Casanova JE, Sligar SG, Schwartz MA. Anal Biochem. 2008 Mar 15. 374(2):243-9. 10.1016/j.ab.2007.11.032 PubMed 18162163