pDR111_mannose_Bgal no NLS
(Plasmid #188399)

• Purpose: Expresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.
• Depositing Lab: Christopher Contag
• Publication: Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi: 10.1038/s42003-022-03851-6.

Backbone

• Vector backbone: pDR111
• Backbone size w/o insert (bp): 8858
• Total vector size (bp): 12092
• Modifications to backbone: Addition of insert and fusion components to NheI site. Phyper-spank replaced by D-mannose promoter and lacI replaced by mannose regulator
• Vector type: Bacterial Expression
• Selectable markers: Spectinomycin for Bacillus subtilis

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: lacZ
• Alt name: Beta-galactosidase
• Species: Escherichia coli
• Insert Size (bp): 3072
• Promoter: D-mannose
• Tag / Fusion Protein:
  • PhoD secretion peptide (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: cggTTATTTTTGACACCAGAC
• 3′ sequencing primer: GCCGGGCGTTTTTTATGCAGgggattattccttgcttttttt

Resource Information

• Supplemental Documents:
  • pdr111_mannose_bgal-no-nls.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry