Skip to main content
Addgene

pDR111_Bgal no NLS
(Plasmid #188397)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188397 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pDR111
  • Backbone size w/o insert (bp) 7841
  • Total vector size (bp) 11074
  • Modifications to backbone
    Addition of insert and fusion components to NheI site
  • Vector type
    Bacterial Expression
  • Selectable markers
    Spectinomycin for Bacillus subtilis

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    lacZ
  • Alt name
    Beta-galactosidase
  • Species
    Escherichia coli
  • Insert Size (bp)
    3072
  • Promoter Phyper spank
  • Tag / Fusion Protein
    • PhoD secretion peptide (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer cggTTATTTTTGACACCAGAC
  • 3′ sequencing primer tcgagggtAAaTGTGAGCaCTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDR111_Bgal no NLS was a gift from Christopher Contag (Addgene plasmid # 188397 ; http://n2t.net/addgene:188397 ; RRID:Addgene_188397)

  • For your References section:

    Engineered endosymbionts that alter mammalian cell surface marker, cytokine and chemokine expression. Madsen CS, Makela AV, Greeson EM, Hardy JW, Contag CH. Commun Biol. 2022 Aug 30;5(1):888. doi: 10.1038/s42003-022-03851-6. 10.1038/s42003-022-03851-6 PubMed 36042261
Related items:
Commonly requested with: