pHR_PGK_SNIPR_hsNotch1 LNR, Notch2 JMD
(Plasmid
#188374)
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PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch1-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 188374 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHR
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePGK_antiCD19_Notch1-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64
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SpeciesSynthetic
Cloning Information
- Cloning method Unknown
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The plasmid was generated using In-fusion cloning (Takara bio) - see the corresponding publication for more details on cloning.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHR_PGK_SNIPR_hsNotch1 LNR, Notch2 JMD was a gift from Kole Roybal (Addgene plasmid # 188374 ; http://n2t.net/addgene:188374 ; RRID:Addgene_188374) -
For your References section:
Modular design of synthetic receptors for programmed gene regulation in cell therapies. Zhu I, Liu R, Garcia JM, Hyrenius-Wittsten A, Piraner DI, Alavi J, Israni DV, Liu B, Khalil AS, Roybal KT. Cell. 2022 Apr 14;185(8):1431-1443.e16. doi: 10.1016/j.cell.2022.03.023. 10.1016/j.cell.2022.03.023 PubMed 35427499