pJJS008
(Plasmid
#188331)
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PurposePCR template for repair templates to tag a gene with a wrmScarlet and mIAA7 AID degron using homology-directed repair for C. elegans.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 188331 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescript SK (+)
- Backbone size w/o insert (bp) 2863
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLinker::wrmScarlet::mIAA7
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SpeciesC. elegans (nematode)
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Insert Size (bp)1140
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer M13 forward
- 3′ sequencing primer M13 reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJJS008 was a gift from Mike Boxem (Addgene plasmid # 188331 ; http://n2t.net/addgene:188331 ; RRID:Addgene_188331) -
For your References section:
The mIAA7 degron improves auxin-mediated degradation in C. elegans. Sepers JJ, Verstappen NHM, Vo AA, Ragle JM, Ruijtenberg S, Ward JD, Boxem M. G3 (Bethesda). 2022 Aug 27. pii: 6677660. doi: 10.1093/g3journal/jkac222. 10.1093/g3journal/jkac222 PubMed 36029236
