pJJS005
(Plasmid #188328)

• Purpose: PCR template for repair templates to tag a gene with a mNeonGreen and mIAA7 AID degron using homology-directed repair for C. elegans.
• Depositing Lab: Mike Boxem
• Publication: Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660. doi: 10.1093/g3journal/jkac222.

Backbone

• Vector backbone: pBluescript SK (+)
• Backbone size w/o insert (bp): 2863
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mIAA7::mNeonGreen::Linker
• Species: C. elegans (nematode)
• Insert Size (bp): 1155

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: M13 forward
• 3′ sequencing primer: M13 reverse

Resource Information

• Supplemental Documents:
  • pJJS005.gb
• A portion of this plasmid was derived from a plasmid made by: mNG origin: pDD346 (Addgene #133311)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry