pLGR002 LGR Cas9 guide vector
(Plasmid
#188320)
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Purpose(Empty Backbone) Universal backbone for CRISPR/Cas9 sgRNA expression
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 188320 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepU6 sgRNA lentiviral
- Backbone size (bp) 9678
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Vector typeMammalian Expression, Lentiviral, CRISPR
- Promoter pU6 5'CMV and EF1-alpha
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Selectable markersPuromycin
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Tag
/ Fusion Protein
- PuroR-T2A-mTagBFP2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.12.22.521524v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLGR002 LGR Cas9 guide vector was a gift from Laralynne Przybyla (Addgene plasmid # 188320 ; http://n2t.net/addgene:188320 ; RRID:Addgene_188320) -
For your References section:
Compact CRISPR genetic screens enabled by improved guide RNA library cloning. Heo SJ, Enriquez LD, Federman S, Chang AY, Mace R, Shevade K, Nguyen P, Litterman AJ, Shafer S, Przybyla L, Chow ED. Genome Biol. 2024 Jan 19;25(1):25. doi: 10.1186/s13059-023-03132-3. 10.1186/s13059-023-03132-3 PubMed 38243310
