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Addgene

pLumi-LgBiT
(Plasmid #188319)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188319 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEN-luxCDABE
  • Backbone manufacturer
    Harry Mobley Lab
  • Backbone size w/o insert (bp) 5310
  • Vector type
    Bacterial Expression, Luciferase, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    LgBiT
  • Species
    Synthetic
  • Insert Size (bp)
    477
  • Promoter sigma 70 promoter

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GGCATAGTATATCGGCATAGTATAATACGA
  • 3′ sequencing primer CATGTGGTCACGCTTTTCGTT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLumi-LgBiT was a gift from Kate Adamala (Addgene plasmid # 188319 ; http://n2t.net/addgene:188319 ; RRID:Addgene_188319)

  • For your References section:

    Expanding luciferase reporter systems for cell-free protein expression. Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP. Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6. 10.1038/s41598-022-15624-6 PubMed 35798760
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