pTXTL-T7max-FLuc
(Plasmid #188311)

• Purpose: Expression of Firefly luciferase via a T7 promoter in TXTL
• Depositing Lab: Kate Adamala
• Publication: Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.

Backbone

• Vector backbone: pCI-T7Max-UTR1-CTerminus8xHis-T500
• Backbone manufacturer: Adamala Lab
• Backbone size w/o insert (bp): 2918
• Vector type: Bacterial Expression, Luciferase, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Firefly luciferase
• Alt name: F-Luc
• Alt name: FLuc
• Species: Photinus pyralis
• Insert Size (bp): 1650
• Promoter: T7Max
• Tag / Fusion Protein:
  • 8x His-tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (not destroyed)
• 3′ cloning site: MluI (not destroyed)
• 5′ sequencing primer: AATAATTTTGTTTAACTTTAAGAAGGAGATATA
• 3′ sequencing primer: GTTGTTGTACAGAAAAGCCCG

Resource Information

• Supplemental Documents:
  • 9-pTXTL-T7maxFLuc-1.gb
  • 9-pTXTL-T7maxFLuc-2.gb
• A portion of this plasmid was derived from a plasmid made by: Michael Axtell Lab

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry