Skip to main content
Addgene

pTXTL-T7max-Fre
(Plasmid #188310)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188310 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCI-T7Max-UTR1-CTerminus8xHis-T500
  • Backbone manufacturer
    Adamala Lab
  • Backbone size w/o insert (bp) 2918
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    fre
  • Species
    Synthetic
  • Insert Size (bp)
    699
  • Promoter T7Max
  • Tag / Fusion Protein
    • 8x His-tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site MluI (not destroyed)
  • 5′ sequencing primer AATAATTTTGTTTAACTTTAAGAAGGAGATATA
  • 3′ sequencing primer GTTGTTGTACAGAAAAGCCCG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTXTL-T7max-Fre was a gift from Kate Adamala (Addgene plasmid # 188310 ; http://n2t.net/addgene:188310 ; RRID:Addgene_188310)

  • For your References section:

    Expanding luciferase reporter systems for cell-free protein expression. Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP. Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6. 10.1038/s41598-022-15624-6 PubMed 35798760
Related items: