pCAG-TcCas12b-T2A-EGFP
(Plasmid
#188277)
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PurposeMammalian expression, Genome editing
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 188277 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTcCas12b
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SpeciesSynthetic
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Mutationnone
- Promoter CAG
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-TcCas12b-T2A-EGFP was a gift from Wei Li (Addgene plasmid # 188277 ; http://n2t.net/addgene:188277 ; RRID:Addgene_188277) -
For your References section:
Artificial sgRNAs engineered for genome editing with new Cas12b orthologs. Teng F, Cui T, Gao Q, Guo L, Zhou Q, Li W. Cell Discov. 2019 Apr 23;5:23. doi: 10.1038/s41421-019-0091-0. eCollection 2019. 10.1038/s41421-019-0091-0 PubMed 31016029