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Addgene

BPK2014-Cas12i2
(Plasmid #188275)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188275 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    BPK2014
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Cas12i2
  • Species
    Synthetic
  • Mutation
    none
  • Promoter T7

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BPK2014-Cas12i2 was a gift from Wei Li (Addgene plasmid # 188275 ; http://n2t.net/addgene:188275 ; RRID:Addgene_188275)

  • For your References section:

    Synergistic engineering of CRISPR-Cas nucleases enables robust mammalian genome editing. Chen Y, Hu Y, Wang X, Luo S, Yang N, Chen Y, Li Z, Zhou Q, Li W. Innovation (Camb). 2022 May 26;3(4):100264. doi: 10.1016/j.xinn.2022.100264. eCollection 2022 Jul 12. 10.1016/j.xinn.2022.100264 PubMed 35693153
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