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Addgene

pCAG-PlmCasX(T26R/K610R/K808R)-T2A-EGFP
(Plasmid #188274)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188274 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PlmCasX(T26R/K610R/K808R)
  • Alt name
    PlmCas12e
  • Species
    Synthetic
  • Mutation
    none
  • Promoter CAG

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-PlmCasX(T26R/K610R/K808R)-T2A-EGFP was a gift from Wei Li (Addgene plasmid # 188274 ; http://n2t.net/addgene:188274 ; RRID:Addgene_188274)

  • For your References section:

    Synergistic engineering of CRISPR-Cas nucleases enables robust mammalian genome editing. Chen Y, Hu Y, Wang X, Luo S, Yang N, Chen Y, Li Z, Zhou Q, Li W. Innovation (Camb). 2022 May 26;3(4):100264. doi: 10.1016/j.xinn.2022.100264. eCollection 2022 Jul 12. 10.1016/j.xinn.2022.100264 PubMed 35693153
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