pCAG-AaCas12b(Q119F/E475R/E758R)-T2A-EGFP Sequences (1)

Addgene Sequences:

Full (1)

Full Sequences from Addgene (1)

Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence).

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GenBank

SnapGene

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> Addgene Partial NGS Result ACGCGTTGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCAT ATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCC ATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTG GAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTG ACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTG GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTCGAGGTGAGCCCCACGTTCTGCTTCACTCTC CCCATCTCCCCCCCCTCCCCACCCCCAATTTTGTATTTATTTATTTTTTAATTATTTTGTGCAGCGATGG GGGCGGGGGGGGGGGGGGCGCGCGCCAGGCGGGGCGGGGCGGGGCGAGGGGCGGGGCGGGGCGAGGCGGA GAGGTGCGGCGGCAGCCAATCAGAGCGGCGCGCTCCGAAAGTTTCCTTTTATGGCGAGGCGGCGGCGGCG GCGGCCCTATAAAAAGCGAAGCGCGCGGCGGGCGGGAGTCGCTGCGCGCTGCCTTCGCCCCGTGCCCCGC TCCGCCGCCGCCTCGCGCCGCCCGCCCCGGCTCTGACTGACCGCGTTACTCCCACAGGTGAGCGGGCGGG ACGGCCCTTCTCCTCCGGGCTGTAATTAGCGCTTGGTTTAATGACGGCTTGTTTCTTTTCTGTGGCTGCG TGAAAGCCTTGAGGGGCTCCGGGAGGGCCCTTTGTGCGGGGGGAGCGGCTCGGGGGGTGCGTGCGTGTGT GTGTGCGTGGGGAGCGCCGCGTGCGGCTCCGCGCTGCCCGGCGGCTGTGAGCGCTGCGGGCGCGGCGCGG GGCTTTGTGCGCTCCGCAGTGTGCGCGAGGGGAGCGCGGCCGGGGGCGGTGCCCCGCGGTGCGGGGGGGG CTGCGAGGGGAACAAAGGCTGCGTGCGGGGTGTGTGCGTGGGGGGGTGAGCAGGGGGTGTGGGCGCGTCG GTCGGGCTGCAACCCCCCCTGCACCCCCCTCCCCGAGTTGCTGAGCACGGCCCGGCTTCGGGTGCGGGGC TCCGTACGGGGCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGGTGGCGGCAGGTGGGGGTGCCGGGCGG GGCGGGGCCGCCTCGGGCCGGGGAGGGCTCGGGGGAGGGGCGCGGCGGCCCCCGGAGCGCCGGCGGCTGT CGAGGCGCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCGTGCGAGAGGGCGCAGGGACTTCCTTTGT CCCAAATCTGTGCGGAGCCGAAATCTGGGAGGCGCCGCCGCACCCCCTCTAGCGGGCGCGGGGCGAAGCG GTGCGGCGCCGGCAGGAAGGAAATGGGCGGGGAGGGCCTTCGTGCGTCGCCGCGCCGCCGTCCCCTTCTC CCTCTCCAGCCTCGGGGCTGTCCGCGGGGGGACGGCTGCCTTCGGGGGGGACGGGGCAGGGCGGGGTTCG GCTTCTGGCGTGTGACCGGCGGCTCTAGCGCCTCTGCTAACCATGTTCATGCCTTCTTCTTTTTCCTACA GCTCCTGGGCAACGTGCTGGTTATTGTGCTGTCTCATCATTTTGGCAAAGCTAGTGAATTCTAATACGAC TCACTATAGGCCGCCACCATGCCCAAGAAGAAGAGGAAGGTTCCCGGGATGGCCGTGAAGTCCATGAAGG TGAAGCTGCGGCTGGATAACATGCCCGAGATCAGAGCCGGCCTGTGGAAGCTGCACACCGAAGTGAATGC CGGCGTGAGGTACTATACAGAGTGGCTGTCTCTGCTGAGACAGGAGAACCTGTACCGGAGAAGCCCCAAT GGCGACGGCGAGCAGGAGTGCTATAAGACCGCCGAGGAGTGTAAGGCAGAGCTGCTGGAGAGGCTGAGGG CACGGCAGGTGGAGAACGGCCACTGCGGCCCTGCCGGCTCCGACGATGAGCTGCTGCAGCTGGCAAGGCA GCTGTACGAGCTGCTGGTGCCACAGGCAATCGGAGCAAAGGGCGATGCACAGTTCATCGCCAGAAAGTTC CTGTCTCCACTGGCAGACAAGGATGCAGTGGGAGGACTGGGAATCGCAAAGGCAGGCAATAAGCCTCGGT GGGTGAGAATGAGGGAGGCAGGAGAGCCAGGATGGGAGGAGGAGAAGGCAAAGGCAGAGGCAAGGAAGAG CACCGACAGGACAGCAGATGTGCTGAGGGCCCTGGCAGACTTCGGCCTGAAGCCACTGATGCGCGTGTAC ACCGACTCCGATATGAGCTCCGTGCAGTGGAAGCCTCTGAGGAAGGGACAGGCCGTGCGGACATGGGACA GAGATATGTTTCAGCAGGCCATCGAGCGGATGATGTCCTGGGAGTCTTGGAACCAGAGAGTGGGCGAGGC CTATGCCAAGCTGGTGGAGCAGAAGAGCCGCTTCGAGCAGAAGAACTTTGTGGGCCAGGAGCACCTGGTG CAGCTGGTGAATCAGCTGCAGCAGGATATGAAGGAGGCCTCTCACGGCCTGGAGAGCAAGGAGCAGACCG CACACTACCTGACAGGACGGGCCCTGAGAGGATCCGACAAGGTGTTCGAGAAGTGGGAGAAGCTGGACCC CGATGCCCCTTTTGACCTGTATGATACCGAGATCAAGAACGTGCAGAGGCGCAATACACGGAGATTCGGC AGCCACGATCTGTTTGCCAAGCTGGCCGAGCCAAAGTACCAGGCCCTGTGGAGGGAGGACGCCTCCTTCC TGACCCGCTACGCCGTGTATAACTCTATCGTGAGGAAGCTGAATCACGCCAAGATGTTCGCCACCTTTAC ACTGCCAGATGCCACCGCCCACCCCATCTGGACACGCTTTGACAAGCTGGGCGGCAACCTGCACCAGTAT ACATTCCTGTTTAATGAGTTCGGAGAGGGCAGGCACGCAATCAGGTTTCAGAAGCTGCTGACCGTGGAGG ACGGAGTGGCAAAGGAGGTGGACGATGTGACAGTGCCCATCTCCATGTCTGCCCAGCTGGACGATCTGCT GCCAAGGGATCCACACGAGCTGGTGGCCCTGTACTTCCAGGACTATGGCGCCGAGCAGCACCTGGCAGGA AGATTTGGAGGAGCCAAGATCCAGTACAGGCGCGATCAGCTGAACCACCTGCACGCAAGGAGAGGAGCAA GAGACGTGTATCTGAATCTGTCCGTGCGGGTGCAGAGCCAGTCCGAGGCAAGAGGAGAGAGGCGCCCCCC TTACGCAGCCGTGTTCCGGCTGGTGGGCGACAACCACAGAGCCTTCGTGCACTTTGACAAGCTGTCCGAT TATCTGGCCGAGCACCCTGACGATGGCAAGCTGGGATCCGAGGGACTGCTGTCTGGACTGAGAGTGATGT CTGTGGATCTGGGCCTGAGAACCTCTGCCAGCATCTCCGTGTTTAGGGTGGCCCGCAAGGACGAGCTGAA GCCTAATAGCGAGGGCCGGGTGCCTTTCTGCTTTCCAATCGAGGGCAACGAGAATCTGGTGGCCGTGCAC GAGAGATCCCAGCTGCTGAAGCTGCCAGGCGAGACAGAGTCTAAGGATCTGAGGGCAATCAGAGAGGAGA GGCAGCGGACCCTGAGGCAGCTGCGCACACAGCTGGCCTACCTGCGGCTGCTGGTGAGATGCGGAAGCGA GGACGTGGGCCGGAGAGAGAGGTCCTGGGCCAAGCTGATCGAGCAGCCTATGGACGCCAACCAGATGACC CCAGATTGGCGCGAGGCCTTCGAGGACGAGCTGCAGAAGCTGAAGAGCCTGTACGGCATCTGTGGCGATA GGGAGTGGACAGAGGCCGTGTATGAGTCCGTGAGGCGCGTGTGGAGGCACATGGGCAAGCAGGTGCGGGA CTGGAGAAAGGATGTGAGGTCTGGAGAGAGGCCAAAGATCAGGGGCTATCAGAAGGACGTGGTGGGCGGC AATAGCATCAGACAGATCGAGTACCTGGAGAGGCAGTATAAGTTCCTGAAGTCTTGGAGCTTCTTTGGCA AGGTGAGCGGCCAGGTCATCAGAGCCGAGAAGGGCTCCAGGTTTGCCATCACCCTGCGCGAGCACATCGA CCACGCCAAGGAGGATAGGCTGAAGAAGCTGGCCGATCGCATCATCATGGAGGCCCTGGGCTACGTGTAT GCCCTGGACGATGAGCGCGGCAAGGGCAAGTGGGTGGCCAAGTACCCACCCTGTCAGCTGATCCTGCTGG AGGAGCTGAGCGAGTATCAGTTCAACAATGACCGGCCTCCATCTGAGAACAATCAGCTGATGCAGTGGAG CCACAGAGGCGTGTTTCAGGAGCTGCTGAACCAGGCCCAGGTGCACGATCTGCTGGTGGGCACCATGTAC GCCGCCTTCTCTAGCAGATTTGACGCAAGGACAGGAGCACCAGGAATCAGGTGCAGGAGAGTGCCTGCAA GGTGTGCAAGGGAGCAGAACCCAGAGCCTTTCCCATGGTGGCTGAATAAGTTTGTGGCCGAGCACAAGCT GGACGGCTGCCCACTGAGAGCAGACGATCTGATCCCAACCGGAGAGGGAGAGTTCTTTGTGAGCCCTTTC TCCGCCGAGGAGGGCGATTTTCACCAGATCCACGCCGACCTGAACGCCGCCCAGAATCTGCAGAGGCGCC TGTGGAGCGACTTCGATATCTCCCAGATCAGACTGAGGTGTGACTGGGGAGAGGTGGATGGAGAGCCCGT GCTGATCCCTAGAACCACAGGCAAGAGGACCGCCGATTCTTACGGCAACAAGGTGTTCTATACCAAGACA GGCGTGACATACTATGAGCGCGAGCGGGGCAAGAAGCGGAGAAAGGTGTTTGCACAGGAGGAGCTGAGCG AGGAGGAGGCAGAGCTGCTGGTGGAGGCAGACGAGGCCAGGGAGAAGTCCGTGGTGCTGATGCGCGATCC ATCTGGCATCATCAACCGCGGCGACTGGACCCGGCAGAAGGAGTTCTGGAGCATGGTGAATCAGAGAATC GAGGGCTACCTGGTGAAGCAGATCAGATCTAGGGTGCGCCTGCAGGAGAGCGCCTGTGAGAATACAGGCG ACATCGCTAGCCCAAAGAAGAAGAGGAAAGTCTCTAGATACCCTTATGATGTTCCAGATTATGCCGGATA CCCATACGATGTCCCTGACTATGCAGGCTCCTACCCTTATGACGTCCCAGACTACGCCGGATCCAGGTCC GGCGGCGGAGAGGGCAGAGGAAGTCTTCTAACATGCGGTGACGTGGAGGAGAATCCCGGCCCAATGGTGA GCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCA CAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGC ACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCA GCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGA GCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACC CTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGG AGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTT CAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATC GGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCA ACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGA GCTGTACAAGTAACTGCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCACCCCAACTTGTTTATTGCA GCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATT CTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATTAATTAACGTCGACCTCTAGCTAGAGCTTGGC GTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCC GGAAGCATAAAGTGTAAAGCCTAGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCAC TGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGG CGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGG CGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGA ACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAG GCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTA TAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCG GATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAG TTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCC TTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTG GTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGG CTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGT AGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGC GCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAA CTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAA TGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTG AGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAAC TACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCT CCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCG CCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAA CGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGT TCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTC CGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCT TACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAG TGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTT TAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATC CAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGG TGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCA TACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGA ATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCGAC GGATCGGGAGATCGATCTCCCGATCCCCTAGGGTCGACTCTCAGTACAATCTGCTCTGATGCCGCATAGT TAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTAC AACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTGCTTAGGGTTAGGCGTTTTGCGCTGCTTCGC GATGTACGGGCCAGATAT

pCAG-AaCas12b(Q119F/E475R/E758R)-T2A-EGFP Sequences (1)

Full Sequences from Addgene (1)

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