nERb446-gs10-NLS-VPR
(Plasmid
#188263)
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PurposePlasmid ensures constitutive expression of the N-terminal fragment of split ERbeta (aa 261-446), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 188263 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namenERb446
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SpeciesH. sapiens (human)
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Entrez GeneESR1 (a.k.a. ER, ESR, ESRA, ESTRR, Era, NR3A1)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
nERb446-gs10-NLS-VPR was a gift from Roman Jerala (Addgene plasmid # 188263 ; http://n2t.net/addgene:188263 ; RRID:Addgene_188263) -
For your References section:
Chemically inducible split protein regulators for mammalian cells. Rihtar E, Lebar T, Lainscek D, Kores K, Lesnik S, Bren U, Jerala R. Nat Chem Biol. 2022 Sep 26. doi: 10.1038/s41589-022-01136-x. 10.1038/s41589-022-01136-x PubMed 36163385
