TRPML1-YFP
(Plasmid #18826)

• Depositing Lab: Craig Montell
• Publication: Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-27.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TRPML1
• Alt name: Mucolipin-1
• Species: H. sapiens (human)
• Insert Size (bp): 1743
• GenBank ID: NM_020533
• Entrez Gene: MCOLN1 (a.k.a. LECD, MG-2, ML1, ML4, MLIV, MST080, MSTP080, TRP-ML1, TRPM-L1, TRPML1)
• Tag / Fusion Protein:
  • YFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: V5

Resource Information

• A portion of this plasmid was derived from a plasmid made by: EST from Invitrogen
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry