pHG5
(Plasmid
#188253)
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Purpose(Empty Backbone) Integrating plasmid contains the SV1 int/attP locus and the kanamycin-resistance gene (aphII) for selection
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 188253 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBF22
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Vector typeBacterial Expression
- Promoter act1p/actII-orf4
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHG5 was a gift from Hong Gao & Maggie Smith (Addgene plasmid # 188253 ; http://n2t.net/addgene:188253 ; RRID:Addgene_188253) -
For your References section:
Use of orthogonal serine integrases to multiplex plasmid conjugation and integration from E. coli into Streptomyces. Gao H, Smith MCM. Access Microbiol. 2021 Dec 8;3(12):000291. doi: 10.1099/acmi.0.000291. eCollection 2021. 10.1099/acmi.0.000291 PubMed 35024553
Map uploaded by the depositor.
