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mRNA-CleanCap-NanoLuc
(Plasmid #188247)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188247 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRC
  • Backbone size w/o insert (bp) 5233
  • Total vector size (bp) 5836
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NanoLuc
  • Species
    Synthetic
  • Insert Size (bp)
    603
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (from mRNA-CleanCap entry vector) (destroyed during cloning)
  • 3′ cloning site BsaI (from mRNA-CleanCap entry vector) (destroyed during cloning)
  • 5′ sequencing primer GGCCAGTGAATTGTAATACGACTCACTATAAG
  • 3′ sequencing primer CAGACTTTATTCAAAGACCACGGGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mRNA-CleanCap-NanoLuc was a gift from Howard Chang (Addgene plasmid # 188247 ; http://n2t.net/addgene:188247 ; RRID:Addgene_188247)

  • For your References section:

    Engineering circular RNA for enhanced protein production. Chen R, Wang SK, Belk JA, Amaya L, Li Z, Cardenas A, Abe BT, Chen CK, Wender PA, Chang HY. Nat Biotechnol. 2022 Jul 18. pii: 10.1038/s41587-022-01393-0. doi: 10.1038/s41587-022-01393-0. 10.1038/s41587-022-01393-0 PubMed 35851375
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