pc-GAKIN-Ha
(Plasmid #188246)

• Purpose: Express GAKIN in mammalian cells with Ha tag
• Depositing Lab: Joel Pomerantz
• Publication: Lamason et al Mol Cell. 2010 Dec 10;40(5):798-809. doi: 10.1016/j.molcel.2010.11.007.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 10944
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GAKIN
• Species: H. sapiens (human)
• Insert Size (bp): 5266
• Entrez Gene: KIF13B (a.k.a. GAKIN)
• Tag / Fusion Protein:
  • Ha (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: CATAGACCCATGAGCTCGCCGGCGGACGAC
• 3′ sequencing primer: AACCGGAAATCCTGGGCCAGCCGGCCGCCTG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Gakin from Kazusa DNA Research Institute in pBluescript II SK9(+) vector.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry