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Addgene

pET27b+R
(Plasmid #188245)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188245 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET27b+
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size (bp) 5413
  • Vector type
    Bacterial Expression
  • Promoter T7 promoter
  • Tags / Fusion Proteins
    • 6xHis tag (N terminal on insert)
    • TEV site (N terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET27b+R was a gift from Yiping Qi (Addgene plasmid # 188245 ; http://n2t.net/addgene:188245 ; RRID:Addgene_188245)

  • For your References section:

    Delivery of CRISPR-Cas12a Ribonucleoprotein Complex for Genome Editing in an Embryogenic Citrus Cell Line. Fang H, Culver JN, Niedz RP, Qi Y. Methods Mol Biol. 2023;2653:153-171. doi: 10.1007/978-1-0716-3131-7_10. 10.1007/978-1-0716-3131-7_10 PubMed 36995625
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