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Addgene

TRPML1-MYC
(Plasmid #18824)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18824 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRPML1
  • Alt name
    Mucolipin-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1743
  • GenBank ID
    NM_020533
  • Entrez Gene
    MCOLN1 (a.k.a. LECD, MG-2, ML1, ML4, MLIV, MST080, MSTP080, TRP-ML1, TRPM-L1, TRPML1)
  • Tag / Fusion Protein
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer V5
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    EST from Ivitrogen

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TRPML1-MYC was a gift from Craig Montell (Addgene plasmid # 18824 ; http://n2t.net/addgene:18824 ; RRID:Addgene_18824)

  • For your References section:

    Lysosomal localization of TRPML3 depends on TRPML2 and the mucolipidosis-associated protein TRPML1. Venkatachalam K, Hofmann T, Montell C. J Biol Chem. 2006 Jun 23. 281(25):17517-27. 10.1074/jbc.M600807200 PubMed 16606612
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