IRES-creERT2-FNF
(Plasmid
#188230)
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PurposeEMCV IRES-cre-ERT2-FRT-Neo*-FRT cassette
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 188230 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneCustom
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Vector typeMammalian Expression, Bacterial Expression, Mouse Targeting, Cre/Lox
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIRES-creERT2-FNF
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SpeciesSynthetic
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byEMCV IRES is from Peter Mombaerts. cre-ERT2 is from Pierre Chambon. FNF is from Addgene #22687.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This IRES-creERT2-FNF cassette was used in the Id1-IRES-creERT2 allele (http://www.informatics.jax.org/allele/MGI:4366863)
Compared to Bochkov and Palmenberg, 2006 (https://doi.org/10.2144/000112243) this EMCV IRES has a 1-bp deletion and an additional base of A ("7A"). The reason for these changes is unclear. These changes were in the electronic sequence file I received. The actual DNA sequence of the IRES was the same as the electronic sequence file. I incorporated this IRES into my plasmids without introducing additional changes. It was more recently tested in driving tdTomato expression (see https://www.addgene.org/185966/) and it was functional. I did not change those bases back and compare whether the expression level is then higher or lower. It may or may not be attenuated compared to the Bochkov and Palmenberg EMCV IRES.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
IRES-creERT2-FNF was a gift from Mario Capecchi (Addgene plasmid # 188230 ; http://n2t.net/addgene:188230 ; RRID:Addgene_188230)