pDH3-PA-GFP
(Plasmid #18819)

• Depositing Lab: Wei-Lih Lee
• Publication: Vorvis et al Yeast. 2008 Sep;25(9):651-9.

Backbone

• Vector backbone: pDH3
• Backbone size w/o insert (bp): 3917
• Vector type: Yeast tagging
• Selectable markers: Gentamicin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Photoactivatable Green Fluorescent Protein
• Alt name: PA-GFP
• Alt name: Photoactivatable GFP
• Species: Aequorea victoria
• Insert Size (bp): 720

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: GTGACACTATAGAACGCG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Patricia Wadsworth, University of Massachusetts Amherst (pIREShyg-PA-GFP-alpha Tubulin)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

U.S. Tulu, et al. (2003). "Peripheral, Non-Centrosome-Associated Microtubules Contribute to Spindle Formation in Centrosome-Containing Cells" Curr Biol 13: 1894-99.