pGL4.23-Fosprom
(Plasmid #188113)

• Purpose: Expresses luciferase under control of Fos promoter in transfected cells
• Depositing Lab: Jennifer Mitchell
• Publication: Shchuka et al PLoS One. 2023 Jan 3;18(1):e0271081. doi: 10.1371/journal.pone.0271081. eCollection 2023.

Backbone

• Vector backbone: pGL4.23
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4283
• Total vector size (bp): 5091
• Modifications to backbone: Minimal promoter removed and replaced with endogenous gene promoter.
• Vector type: Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Fos promoter
• Alt name: cFos
• Species: M. musculus (mouse)
• Insert Size (bp): 901
• Entrez Gene: Fos (a.k.a. D12Rfj1, c-fos, cFos)
• Promoter: Fos endogenous promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: NcoI (not destroyed)
• 5′ sequencing primer: ACTTATTTACAATCCTTCACTTGCT
• 3′ sequencing primer: GGTCGAAGTTTGGGGAAAGCC

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Cloning by Virlana M Shchuka.