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Addgene

pN1-RnKIF5C(1-560,Δ6)-Halo-Flag
(Plasmid #188072)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188072 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    rat KIF5C
  • Species
    R. norvegicus (rat)
  • Mutation
    aa1-560, deletion of aa2-6
  • Entrez Gene
    Kif5c
  • Promoter CMV
  • Tags / Fusion Proteins
    • Halo (C terminal on insert)
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer EGFPN1seqfor
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pN1-RnKIF5C(1-560,Δ6)-Halo-Flag was a gift from Kristen Verhey (Addgene plasmid # 188072 ; http://n2t.net/addgene:188072 ; RRID:Addgene_188072)

  • For your References section:

    A kinesin-1 variant reveals motor-induced microtubule damage in cells. Budaitis BG, Badieyan S, Yue Y, Blasius TL, Reinemann DN, Lang MJ, Cianfrocco MA, Verhey KJ. Curr Biol. 2022 Jun 6;32(11):2416-2429.e6. doi: 10.1016/j.cub.2022.04.020. Epub 2022 May 2. 10.1016/j.cub.2022.04.020 PubMed 35504282
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