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pJRoC30_Th3.14
(Plasmid #188068)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188068 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJRoC30
  • Backbone manufacturer
    California Institute of Technology
  • Backbone size w/o insert (bp) 10462
  • Total vector size (bp) 12286
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FuncLib-designed high-redox potential laccase from Trametes hirsuta
  • Alt name
    Th3.14
  • Species
    Trametes hirsuta
  • Insert Size (bp)
    1824
  • Mutation
    20 PROSS + 4 FuncLib mutations, described in publication
  • GenBank ID
    EU492907.1 EU492907.1
  • Tags / Fusion Proteins
    • S. cerevisiae evolved α-factor prepro-leader signal peptide (N terminal on insert)
    • Thrombin cleavage site + 6xHis tag (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The pJRoC30 plasmid was provided to the Fleishman lab by EvoEnzyme S.L. via an MTA agreement.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJRoC30_Th3.14 was a gift from Sarel Fleishman (Addgene plasmid # 188068 ; http://n2t.net/addgene:188068 ; RRID:Addgene_188068)

  • For your References section:

    Designed High-Redox Potential Laccases Exhibit High Functional Diversity. Barber-Zucker S, Mateljak I, Goldsmith M, Kupervaser M, Alcalde M, Fleishman SJ. ACS Catal. 2022 Nov 4;12(21):13164-13173. doi: 10.1021/acscatal.2c03006. Epub 2022 Oct 13. 10.1021/acscatal.2c03006 PubMed 36366766
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