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pJRoC30_VP11H
(Plasmid #188063)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188063 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJRoC30
  • Backbone manufacturer
    California Institute of Technology
  • Backbone size w/o insert (bp) 10461
  • Total vector size (bp) 11751
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PROSS-designed versatile peroxidase from Pleurotus ostreatus
  • Alt name
    VP_11H
  • Species
    Pleurotus ostreatus
  • Insert Size (bp)
    1290
  • Mutation
    38 PROSS mutations, described in publication
  • GenBank ID
    ASU87523.1 ASU87523.1
  • Tag / Fusion Protein
    • S. cerevisiae native α-factor prepro-leader signal peptide (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The designed VP sequence is patented.
The pJRoC30 plasmid was provided to the Fleishman lab by EvoEnzyme S.L. via an MTA agreement.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJRoC30_VP11H was a gift from Sarel Fleishman (Addgene plasmid # 188063 ; http://n2t.net/addgene:188063 ; RRID:Addgene_188063)

  • For your References section:

    Stable and Functionally Diverse Versatile Peroxidases Designed Directly from Sequences. Barber-Zucker S, Mindel V, Garcia-Ruiz E, Weinstein JJ, Alcalde M, Fleishman SJ. J Am Chem Soc. 2022 Mar 2;144(8):3564-3571. doi: 10.1021/jacs.1c12433. Epub 2022 Feb 18. 10.1021/jacs.1c12433 PubMed 35179866
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