NSUN6
(Plasmid #188060)

• Purpose: Bacterial expression plasmid for human NSUN6; for activity testing
• Depositing Lab: Tanja Schirmeister
• Publication: Schwickert et al J Med Chem. 2022 Jul 18. doi: 10.1021/acs.jmedchem.2c00388.

Backbone

• Vector backbone: pET-22b(+)
• Total vector size (bp): 6773
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: NSUN6
• Alt name: tRNA (cytosine(72)-C(5))-methyltransferase NSUN6
• Alt name: NOP2/Sun RNA methyltransferase 6
• Species: H. sapiens (human)
• Insert Size (bp): 1416
• GenBank ID: 221078
• Entrez Gene: NSUN6 (a.k.a. 4933414E04Rik, ARL5B-AS1, NOPD1)
• Promoter: T7 promoter
• Tag / Fusion Protein:
  • His-tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: T7 primer
• 3′ sequencing primer: T7 terminator reverse primer

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry