pBad-mCherry-XL (R125K)
(Plasmid #188054)

• Purpose: Expresses His-tagged, Arabinose-inducible mCherry-XL (R125K) in bacteria
• Depositing Lab: Ralph Jimenez
• Publication: Mukherjee et al J Phys Chem B. 2022 Jun 16. doi: 10.1021/acs.jpcb.2c01956.

Backbone

• Vector backbone: pBad His+
• Backbone manufacturer: ThermoFisher
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 4700
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Induced expression with 0.1% arabinose
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: mCherry-XL (R125K)
• Species: Synthetic
• Insert Size (bp): 708
• Mutation: differs from mCherry by R125K, W143S, I161V, Q163Y and I197R
• Promoter: araBAD
• Tag / Fusion Protein:
  • 6xHis (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: QB0068 (Quintara Bio)
• 3′ sequencing primer: QB0069 (Quintara Bio)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: The amino acid numbering of mutations is based on the mCherry crystal structure (PDB: 2H5Q), which is missing the first five amino acids.