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Addgene

pBad-mCherry-XL (R125K)
(Plasmid #188054)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188054 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBad His+
  • Backbone manufacturer
    ThermoFisher
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 4700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Induced expression with 0.1% arabinose
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mCherry-XL (R125K)
  • Species
    Synthetic
  • Insert Size (bp)
    708
  • Mutation
    differs from mCherry by R125K, W143S, I161V, Q163Y and I197R
  • Promoter araBAD
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer QB0068 (Quintara Bio)
  • 3′ sequencing primer QB0069 (Quintara Bio)
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: The amino acid numbering of mutations is based on the mCherry crystal structure (PDB: 2H5Q), which is missing the first five amino acids.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBad-mCherry-XL (R125K) was a gift from Ralph Jimenez (Addgene plasmid # 188054 ; http://n2t.net/addgene:188054 ; RRID:Addgene_188054)
  • For your References section:

    Directed Evolution of a Bright Variant of mCherry: Suppression of Nonradiative Decay by Fluorescence Lifetime Selections. Mukherjee S, Manna P, Hung ST, Vietmeyer F, Friis P, Palmer AE, Jimenez R. J Phys Chem B. 2022 Jun 16. doi: 10.1021/acs.jpcb.2c01956. 10.1021/acs.jpcb.2c01956 PubMed 35709514