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pAG8Ha-EWS_RRM-RGG(359-513)
(Plasmid #188046)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188046 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET11
  • Backbone size w/o insert (bp) 4500
  • Total vector size (bp) 5022
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EWSR1
  • Alt name
    RNA binding protein EWS
  • Alt name
    EWS
  • Alt name
    Ewing sarcoma breakpoint region 1 protein
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    522
  • Mutation
    deleted amino acids 1-358, 514-656
  • Entrez Gene
    EWSR1 (a.k.a. EWS, EWS-FLI1, bK984G1.4)
  • Promoter T7
  • Tag / Fusion Protein
    • His8 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KasI (unknown if destroyed)
  • 3′ cloning site BamH1 (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

insert is codon optimized for E. coli expression

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAG8Ha-EWS_RRM-RGG(359-513) was a gift from David Libich (Addgene plasmid # 188046 ; http://n2t.net/addgene:188046 ; RRID:Addgene_188046)

  • For your References section:

    Biochemical and biophysical characterization of the nucleic acid binding properties of the RNA/DNA binding protein EWS. Selig EE, Bhura R, White MR, Akula S, Hoffman RD, Tovar CN, Xu X, Booth RE, Libich DS. Biopolymers. 2023 May;114(5):e23536. doi: 10.1002/bip.23536. Epub 2023 Mar 17. 10.1002/bip.23536 PubMed 36929870
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