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CapChR2_pUAST-attb
(Plasmid #188041)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188041 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUAST-attb
  • Backbone size w/o insert (bp) 8500
  • Total vector size (bp) 9336
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CapChR2
  • Alt name
    Calcium permeable Channelrhodopsin 2
  • Species
    Synthetic; Chloromonas oogama
  • Insert Size (bp)
    867
  • Mutation
    S43D, L112C, T139C and N238E mutations in CoChR
  • Promoter UAS
  • Tag / Fusion Protein
    • none

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: LoxP is in the opposite orientation compared to the depositor's provided sequence. It is not known how this change affects plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CapChR2_pUAST-attb was a gift from Peter Hegemann (Addgene plasmid # 188041 ; http://n2t.net/addgene:188041 ; RRID:Addgene_188041)

  • For your References section:

    Calcium-permeable channelrhodopsins for the photocontrol of calcium signalling. Fernandez Lahore RG, Pampaloni NP, Peter E, Heim MM, Tillert L, Vierock J, Oppermann J, Walther J, Schmitz D, Owald D, Plested AJR, Rost BR, Hegemann P. Nat Commun. 2022 Dec 21;13(1):7844. doi: 10.1038/s41467-022-35373-4. 10.1038/s41467-022-35373-4 PubMed 36543773
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