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Addgene

CapChR1_pUAST-attb
(Plasmid #188040)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188040 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUAST-attb
  • Backbone size w/o insert (bp) 8000
  • Total vector size (bp) 9378
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CapChR1
  • Alt name
    Calcium permeable Channelrhodopsin 1
  • Species
    Synthetic; Chlamydomonas reinhardtii
  • Insert Size (bp)
    933
  • Mutation
    S63D, L132C, T159C and N258E mutations in CrChR2
  • Promoter UAS
  • Tag / Fusion Protein
    • none

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CapChR1_pUAST-attb was a gift from Peter Hegemann (Addgene plasmid # 188040 ; http://n2t.net/addgene:188040 ; RRID:Addgene_188040)

  • For your References section:

    Calcium-permeable channelrhodopsins for the photocontrol of calcium signalling. Fernandez Lahore RG, Pampaloni NP, Peter E, Heim MM, Tillert L, Vierock J, Oppermann J, Walther J, Schmitz D, Owald D, Plested AJR, Rost BR, Hegemann P. Nat Commun. 2022 Dec 21;13(1):7844. doi: 10.1038/s41467-022-35373-4. 10.1038/s41467-022-35373-4 PubMed 36543773
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