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Addgene

pWZL Blast mouse E-cadherin
(Plasmid #18804)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18804 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pWZL-Blast
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    E-cadherin
  • Alt name
    E-Cadherin
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Cdh1 (a.k.a. ARC-1, E-cad, Ecad, L-CAM, UVO, Um)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer pBABE 5'
  • 3′ sequencing primer IRES-R (5'-GCATTCCTTTGGCGAGAG-3')
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    E-cadherin cDNA provided by Dr. Gerhard Christofori
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Full-length murine E-cadherin cDNA cloned into pWZL blast.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWZL Blast mouse E-cadherin was a gift from Bob Weinberg (Addgene plasmid # 18804 ; http://n2t.net/addgene:18804 ; RRID:Addgene_18804)

  • For your References section:

    Loss of E-cadherin promotes metastasis via multiple downstream transcriptional pathways. Onder TT, Gupta PB, Mani SA, Yang J, Lander ES, Weinberg RA. Cancer Res. 2008 May 15. 68(10):3645-54. 10.1158/0008-5472.CAN-07-2938 PubMed 18483246
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