pAAV-Syn_CapChR1_eYFP
(Plasmid #188033)

• Purpose: Expresses CapChR1-eYFP in mammalian neurons
• Depositing Lab: Peter Hegemann
• Publication: Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi: 10.1038/s41467-022-35373-4.

Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 6354
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CapChR1-eYFP
• Alt name: Calcium permeable Channelrhodopsin 1
• Species: Synthetic; Chlamydomonas reinhardtii
• Insert Size (bp): 1656
• Mutation: S63D, L132C, T159C and N258E mutations in CrChR2
• Promoter: hSyn
• Tag / Fusion Protein:
  • eYFP (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: hSyn-F
• 3′ sequencing primer: WPRE-R

Resource Information

• Supplemental Documents:
  • 3_pAAV-Syn_CapChR1_YFP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Plasmid contains a single C to A nucleotide mutation in the AAV2 ITR. It is not known how this mutation affects plasmid function.