CapChR1_pmCherry-N1
(Plasmid #188031)

• Purpose: Expresses CapChR1-mCherry in mammalian cells
• Depositing Lab: Peter Hegemann
• Publication: Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi: 10.1038/s41467-022-35373-4.

Backbone

• Vector backbone: pN1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4012
• Total vector size (bp): 5659
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CapChR1-mCherry
• Alt name: Calcium permeable Channelrhodopsin 1
• Species: Synthetic; Chlamydomonas reinhardtii
• Insert Size (bp): 1647
• Mutation: S63D, L132C, T159C and N258E mutations in CrChR2
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP-C1-R

Resource Information

• Supplemental Documents:
  • 1_CapChR1_pmCherry-N1.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry