pWZL Blast DN E-cadherin
(Plasmid #18800)

• Depositing Lab: Bob Weinberg
• Publication: Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

Backbone

• Vector backbone: pWZL-Blast
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Dom neg E-cadherin
• Alt name: E-Cadherin
• Species: M. musculus (mouse)
• Mutation: truncated form of E-cadherin (dominant negative) lacking the ectodomain of the wild-type protein
• Entrez Gene: Cdh1 (a.k.a. ARC-1, E-cad, Ecad, L-CAM, UVO, Um)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pBABE 5'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: E-cadherin cDNA provided by Dr. Gerhard Christofori
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Truncated murine E-cadherin cDNA cloned into pWZL blast.