pGBdest-GST-PI3Kc1
(Plasmid #187992)

• Purpose: Plasmid for the protein expression of GST-3C-ATG14/Vps34/Vps15/BECN1 in a bacterial and insect expression system. Internal_Reference: SMC1326
• Depositing Lab: Sascha Martens
• Publication: ASAP - PI3KC3-C1 related projects (unpublished)

Backbone

• Vector backbone: pGB-08-09
• Backbone manufacturer: Protech Facilities VBCF, Vienna, Austria
• Backbone size w/o insert (bp): 4232
• Total vector size (bp): 16481
• Vector type: Bacterial Expression, Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: ATG14
• Species: H. sapiens (human)
• Insert Size (bp): 2886
• GenBank ID: 22863
• Entrez Gene: ATG14 (a.k.a. ATG14L, BARKOR, KIAA0831)
• Tag / Fusion Protein:
  • GST (N terminal on insert)

Gene/Insert 2

• Gene/Insert name: VPS34
• Species: H. sapiens (human)
• Insert Size (bp): 2664
• GenBank ID: 5289
• Entrez Gene: PIK3C3 (a.k.a. VPS34, Vps34, hVps34)

Gene/Insert 3

• Gene/Insert name: VPS15
• Species: H. sapiens (human)
• Insert Size (bp): 2664
• GenBank ID: 30849
• Entrez Gene: PIK3R4 (a.k.a. VPS15, p150)

Gene/Insert 4

• Gene/Insert name: BECN1
• Alt name: beclin1
• Species: H. sapiens (human)
• Insert Size (bp): 1353
• GenBank ID: 8678
• Entrez Gene: BECN1 (a.k.a. ATG6, VPS30, beclin1)

Resource Information

• Supplemental Documents:
  • SMC1326 - 631_S_0_1385_3SM014-PI3Kc1.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry