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pWZL Blast Twist ER
(Plasmid #18799)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18799 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pWZL-Blast
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    twist
  • Species
    M. musculus (mouse)
  • Mutation
    G525R mutation in the ER. The presence of the G525R point mutation in the cloned ER sequence abrogates its binding to 17β-estradiol while keeping its full sensitivity to the synthetic ligand 4'hydroxytamoxifen (4OHT)
  • Entrez Gene
    Twist1 (a.k.a. M-Twist, Pde, Ska10, Ska<m10Jus>, Twist, bHLHa38, pdt)
  • Tag / Fusion Protein
    • ER hormone binding domain (C terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer pBABE 5'
  • 3′ sequencing primer IRES-R (5'-GCATTCCTTTGGCGAGAG-3')
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pWZL-Blast-Twist-ER was
generated by replacing the MYC cDNA of pWZL-Blast-DN-MycER
(Littlewood et al., 1995; Watnick et al., 2003) with the mTwist cDNA PCR amplified from pBp-mTwist.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWZL Blast Twist ER was a gift from Bob Weinberg (Addgene plasmid # 18799 ; http://n2t.net/addgene:18799 ; RRID:Addgene_18799)

  • For your References section:

    The epithelial-mesenchymal transition generates cells with properties of stem cells. Mani SA, Guo W, Liao MJ, Eaton EN, Ayyanan A, Zhou AY, Brooks M, Reinhard F, Zhang CC, Shipitsin M, Campbell LL, Polyak K, Brisken C, Yang J, Weinberg RA. Cell. 2008 May 16. 133(4):704-15. 10.1016/j.cell.2008.03.027 PubMed 18485877
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