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pGB-05-06-GST-ATG14
(Plasmid #187987)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187987 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGB-05-06
  • Backbone manufacturer
    Protech Facilities VBCF, Vienna, Austria
  • Backbone size w/o insert (bp) 3619
  • Total vector size (bp) 5098
  • Vector type
    Bacterial Expression, Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ATG14
  • Alt name
    Barkor
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1479
  • GenBank ID
    22863 NM_014924
  • Entrez Gene
    ATG14 (a.k.a. ATG14L, BARKOR, KIAA0831)
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer tcCcggtccgaaaccatgtcccctatactag
  • 3′ sequencing primer TCCCCAGAACATCAGGTTAATGGCGTTACCTATGTCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGB-05-06-GST-ATG14 was a gift from Sascha Martens (Addgene plasmid # 187987 ; http://n2t.net/addgene:187987 ; RRID:Addgene_187987)
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