mCherry-p62-Silent_Mutation_WT
(Plasmid #187986)

• Purpose: human p62 with silent mutation for sip62 #5 (Thermo Scientific). Internal reference: SMC516
• Depositing Lab: Sascha Martens
• Publication: Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.7554/eLife.08941.

Backbone

• Vector backbone: pmCherry-C1 Vector
• Backbone manufacturer: Clontech #632524
• Backbone size w/o insert (bp): 4722
• Total vector size (bp): 6013
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: p62
• Species: H. sapiens (human)
• Mutation: silent mutation for resistance to sip62 #5
• GenBank ID: 23636 NM_153719
• Entrez Gene: NUP62 (a.k.a. IBSN, SNDI, p62)
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: ATGGTGAGCAAGGGCGAGGAGGATA
• 3′ sequencing primer: gggTCACAACGGCGGGGGATGCTTTGAATA

Resource Information

• Supplemental Documents:
  • SMc516_mCherry-p62 sil_DomainsSelect.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry